ATP6V1A Antibody

Western Blot: ATP6V1A Antibody [NBP1-33021]

Western Blot: ATP6V1A Antibody [NBP1-33021] - Various whole cell extracts (30ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody diluted by 1:10000.

Immunoprecipitation: ATP6V1A Antibody [NBP1-33021]

Immunoprecipitation: ATP6V1A Antibody [NBP1-33021] - ATP6V1A Antibody [NBP1-33021] - Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 ug of ATP6V1A antibody. Western blot analysis was performed using ATP6V1A antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Knockout Validated: ATP6V1A Antibody [NBP1-33021]

Knockout Validated: ATP6V1A Antibody [NBP1-33021] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ATP6V1A Antibody [NBP1-33021] -

Western Blot: ATP6V1A Antibody [NBP1-33021] - Functional analysis of the vATPase on CCVs.(A) Immunoblots of isolated SVs & CCVs for the clathrin light (LC) & heavy chains (HC), & SV marker proteins synaptophysin (Syph) & VAMP2 (left panel), as well as various Vo & V1 subunits of vATPase (right panel). (B) The ratio of the Vo & V1 as well as synaptophysin & VAMP2 (as SV markers) detected by immunoblotting in equal protein amount of CCVs & SVs (C) Normalized levels of Vo & V1 in CCV & SV samples, indicating that Vo:V1 ratio is 1:1 in both preparations. (D) ATPase activity measured in 1.3 µg of isolated SVs & CCVs. (E) Blocking percentage of ATPase activity by NEM (vATPase inhibitor) in 1.3 µg of SVs & CCVs. Error bars in (B–E) represent SD of 3–4 experimental replicates (p<0.01 for D & E, and >0.05 for C).Both Vo & V1 subunits are present on CCVs.Immunoblot for Voa & V1A subunits indicate presence of subunits during the CCV isolation procedure.ATPase activity measurements in isolated SVs.To make sure that the measured ATPase activity is within the standard curve range of the used kit, we tested different amounts of isolated SVs. The measurements of ATPase activity were then performed with 1.3 µg of SVs & CCVs which is in the liner range (inset). Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/32569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.