ATP7b Antibody (3E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00000540-M01

Novus Biologicals, part of Bio-Techne
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H00000540-M01
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Key Product Details

Species Reactivity

Human, Rat, Canine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP7b Antibodies »

Product Specifications

Immunogen

ATP7B (NP_000044, 1372 a.a. ~ 1465 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLKCYKKPDLERYEAQAHGHMKPLTASQVSVHIGMDDRWRDSPRATPWDQVSYVSQVSLSSLTSDKPSRHSAAADDDGDKWSLLLNGRDEEQYI

Localization

Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140 kDa is located in mitochondria.

Specificity

ATP7B - ATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ATP7b Antibody (3E10)

Western Blot: ATP7b Antibody (3E10) [H00000540-M01]

Western Blot: ATP7b Antibody (3E10) [H00000540-M01]

Western Blot: ATP7b Antibody (3E10) [H00000540-M01] - ATP7B monoclonal antibody (M01), clone 3E10. Analysis of ATP7B expression in human colon.
ELISA: ATP7b Antibody (3E10) [H00000540-M01]

ELISA: ATP7b Antibody (3E10) [H00000540-M01]

ELISA: ATP7b Antibody (3E10) [H00000540-M01] - ATP7b Antibody (3E10) [H00000540-M01] - Detection limit for recombinant GST tagged ATP7B is approximately 0.3ng/ml as a capture antibody.

Applications for ATP7b Antibody (3E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATP7b

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).

Alternate Names

ATPase, Cu(2+)- transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, copper-transporting ATPase 2, EC 3.6.3, EC 3.6.3.4, PWD, WC1, WD, Wilson disease-associated protein, WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Entrez Gene IDs

540 (Human)

Gene Symbol

ATP7B

OMIM

277900 (Human)

UniProt

NP_000044 (Human)

Additional ATP7b Products

Product Documents for ATP7b Antibody (3E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP7b Antibody (3E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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