ATP7b Antibody

Immunocytochemistry/ Immunofluorescence: ATP7b Antibody [NB100-361]

Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATP7b Antibody NB100-361 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: ATP7b Antibody [NB100-361] -

Western Blot: ATP7b Antibody [NB100-361] - Effects & mechanism of action of CASIN on melphalan-resistant MM cells. (A) CASIN reduces Cdc42 activity (Cdc42-GTP) but not Rac1 activity (Rac1-GTP). Melphalan-sensitive MM cells (S) were treated with Vehicle or CASIN (5 μM) for 8 h. Cdc42 & Rac1 activities were measured using pull-down assay. (B) CASIN (5 μM) inhibits proliferation of both S & melphalan-resistant (LR5) MM cells. **P < 0.01 vs. S-Vehicle; ##P < 0.01 vs. LR5-Vehicle. (C) CASIN (5 μM) preferentially causes apoptosis of melphalan-resistant MM cells. S & LR5 cells were treated with or without CASIN or melphalan (Mel, 25 μM) or both for 2 days. Annexin V+ cells were analyzed using flow cytometry. **P < 0.01. (D–F) Mechanism of action of CASIN on melphalan-resistant MM cells. (D) Cdc42 activity & expression are increased in LR5 cells compared to that in S cells. Cdc42 activity was measured using pull-down assay. beta-Actin was used as loading control. Vertical lines indicate the gel lanes being switched in position from the original blots. (E) CASIN (5 μM) decreases melphalan-induced FANCD2 mono-ubiquitination (Ub-FANCD2) in LR5 but not in S cells. S & LR5 cells were treated with or without CASIN, Mel (25 μM), or both for 16 h. FANCD2 was detected using western blotting. beta-Actin was used as loading control. (F) CASIN sensitizes melphalan-resistant but not -sensitive MM cells to melphalan-induced DNA damage. gamma H2A histone family member X-positive ( gammaH2AX+) cells were detected using flow cytometry. **P < 0.01. Error bars represent means ± SD of triplicates & data are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31632904), licensed under a CC-BY license. Not internally tested by Novus Biologicals.