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ATPase Inhibitory Factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90069

Novus Biologicals, part of Bio-Techne
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NBP1-90069

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VRTMQARGFGSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22971420)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATPase Inhibitory Factor 1 Antibody

Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP1-90069]

Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP1-90069]

Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP1-90069] - Analysis in human heart tissue.
Immunocytochemistry/ Immunofluorescence: ATPase Inhibitory Factor 1 Antibody [NBP1-90069]

Immunocytochemistry/ Immunofluorescence: ATPase Inhibitory Factor 1 Antibody [NBP1-90069]

Immunocytochemistry/Immunofluorescence: ATPase Inhibitory Factor 1 Antibody [NBP1-90069] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP1-90069]

Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP1-90069]

Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP1-90069] - Staining of human testis shows strong cytoplasmic granular positivity in cells in seminiferous ducts.

Applications for ATPase Inhibitory Factor 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATPase Inhibitory Factor 1

ATPase Inhibitory Factor 1 encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.

Alternate Names

ATP synthase inhibitor protein, ATPase inhibitor protein, ATPase inhibitor, mitochondrial, ATPase inhibitory factor 1, ATPIIF(1), ATPIP, IF1, Inhibitor of F(1)F(o)-ATPase, IP, MGC1167, MGC8898

Gene Symbol

ATP5IF1

Additional ATPase Inhibitory Factor 1 Products

Product Documents for ATPase Inhibitory Factor 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATPase Inhibitory Factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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