ATPase Inhibitory Factor 1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92891
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1). GSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATPase Inhibitory Factor 1 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891]](https://resources.bio-techne.com/images/products/ATPase-Inhibitory-Factor-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92891-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891]")
Immunocytochemistry/ Immunofluorescence: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891]
Immunocytochemistry/Immunofluorescence: ATPase Inhibitory Factor 1 Antibody [NBP2-92891] - Analysis of U2OS cells using ATPase Inhibitory Factor 1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891] -
Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891] - Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3 ug [KO Validated] ATPase Inhibitory Factor 1 Rabbit pAb . Western blot was performed from the immunoprecipitate using [KO Validated] ATPase Inhibitory Factor 1 Rabbit pAb at a dilition of 1:3000.
Western Blot: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891] -
Western Blot: ATPase Inhibitory Factor 1 Antibody - BSA Free [NBP2-92891] - Western blot analysis of lysates from wild type(WT) and ATPase Inhibitory Factor 1 knockout (KO) 293T(KO) cells, using [KO Validated] ATPase Inhibitory Factor 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for ATPase Inhibitory Factor 1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunoprecipitation
1:1000 - 1:5000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ATPase Inhibitory Factor 1
Alternate Names
ATP synthase inhibitor protein, ATPase inhibitor protein, ATPase inhibitor, mitochondrial, ATPase inhibitory factor 1, ATPIIF(1), ATPIP, IF1, Inhibitor of F(1)F(o)-ATPase, IP, MGC1167, MGC8898
Gene Symbol
ATP5IF1
Additional ATPase Inhibitory Factor 1 Products
Product Documents for ATPase Inhibitory Factor 1 Antibody - BSA Free
Product Specific Notices for ATPase Inhibitory Factor 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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