ATPase Inhibitory Factor 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99177
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ATPase Inhibitory Factor 1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ATPase Inhibitory Factor 1 Antibody
Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]
Western Blot: ATPase Inhibitory Factor 1 Antibody [NBP2-99177] - Anti-ATPase Inhibitory Factor 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 15 kDa (We are unsure as to the identity of these extra bands).Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]
Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP2-99177] - Immunochemical staining of human ATPase Inhibitory Factor 1 in human thyroid cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody [NBP2-99177]
Immunoprecipitation: ATPase Inhibitory Factor 1 Antibody [NBP2-99177] - ATPase Inhibitory Factor 1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-ATPase Inhibitory Factor 1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ATPase Inhibitory Factor 1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDaApplications for ATPase Inhibitory Factor 1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATPase Inhibitory Factor 1
Alternate Names
ATP synthase inhibitor protein, ATPase inhibitor protein, ATPase inhibitor, mitochondrial, ATPase inhibitory factor 1, ATPIIF(1), ATPIP, IF1, Inhibitor of F(1)F(o)-ATPase, IP, MGC1167, MGC8898
Gene Symbol
ATP5IF1
Additional ATPase Inhibitory Factor 1 Products
Product Documents for ATPase Inhibitory Factor 1 Antibody
Product Specific Notices for ATPase Inhibitory Factor 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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