ATPase Na+/K+ beta 3 Antibody (2F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00000483-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Bovine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATPase Na+/K+ beta 3 Antibodies »

Product Specifications

Immunogen

ATP1B3 (AAH11835, 1 a.a. ~ 279 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTKNEKKSLNQSLAEWKLFIYNPTTGEFLGRTAKSWGLILLFYLVFYGFLAALFSFTMWVMLQTLNDEVPKYRDQIPSPGLMVFPKPVTALEYTFSRSDPTSYAGYIEDLKKFLKPYTLEEQKNLTVCPDGALFEQKGPVYVACQFPISLLQACSGMNDPDFGYSQGNPCILVKMNRIIGLKPEGVPRIDCVSKNEDIPNVAVYPHNGMIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVECKIDGSANLKSQDDRDKFLGRVMFKITARA

Specificity

ATP1B3 - ATPase, Na+/K+ transporting, beta 3 polypeptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ATPase Na+/K+ beta 3 Antibody (2F4)

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05]

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05]

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 3ng/ml as a capture antibody.
ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05]

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05]

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 0.03ng/ml as a capture antibody.

Applications for ATPase Na+/K+ beta 3 Antibody (2F4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control. Use in Western blot reported in scientific literature (PMID 28134274).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATPase Na+/K+ beta 3

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.

Long Name

Sodium/potassium-transporting ATPase subunit beta-3

Alternate Names

ATP1B3, ATPB-3, CD antigen CD298

Entrez Gene IDs

483 (Human)

Gene Symbol

ATP1B3

OMIM

601867 (Human)

UniProt

AAH11835 (Human)

Additional ATPase Na+/K+ beta 3 Products

Product Documents for ATPase Na+/K+ beta 3 Antibody (2F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATPase Na+/K+ beta 3 Antibody (2F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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