ATPB Antibody (OTI4E5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70224

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4E5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ATPB Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATPB Antibody (OTI4E5) - Azide and BSA Free

Western Blot: ATPB Antibody (OTI4E5)Azide and BSA Free [NBP2-70224]

Western Blot: ATPB Antibody (OTI4E5)Azide and BSA Free [NBP2-70224]

Western Blot: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATPB.
Immunocytochemistry/ Immunofluorescence: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224]

Immunocytochemistry/ Immunofluorescence: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224]

Immunocytochemistry/Immunofluorescence: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATPB.
Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224]

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224]

Immunohistochemistry: ATPB Antibody (OTI4E5) - Azide and BSA Free [NBP2-70224] - Staining of paraffin-embedded Ovary tissue using anti-ATPB mouse monoclonal antibody.
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Applications for ATPB Antibody (OTI4E5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATPB

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Alternate Names

ATP synthase subunit beta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATPMB, ATPSBmitochondrial ATP synthase beta subunit, EC 3.6.3, EC 3.6.3.14, MGC5231, mitochondrial ATP synthetase, beta subunit

Gene Symbol

ATP5F1B

Additional ATPB Products

Product Documents for ATPB Antibody (OTI4E5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATPB Antibody (OTI4E5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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