Atrial Natriuretic Peptide/ANP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97752

Novus Biologicals, part of Bio-Techne
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NBP1-97752
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Atrial Natriuretic Peptide/ANP Antibodies »

Product Specifications

Immunogen

Peptide with sequence RIGAQSGLGCNSFR corresponding to C-Terminus according to NP_006163.1.

Specificity

The immunizing peptide represents aa137-150 of the precursor NPPA.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Atrial Natriuretic Peptide/ANP Antibody

Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752]

Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752]

Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Analysis of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752]

Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752]

Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Atrial Natriuretic Peptide/ANP Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/mL

Immunohistochemistry-Paraffin

5 - 10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 19 kDa band observed in mouse and rat heart lysates (calculated MW of 16.4 kDa band according to human precursor NP_006163.1 and 16.6 kDa band accordiong to mouse precursor NP_032751.1). IHC-P: Human heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Atrial Natriuretic Peptide/ANP

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Alternate Names

ANF, ANP, Cardionatrin, NPPA, PND

Entrez Gene IDs

4878 (Human); 230899 (Mouse); 24602 (Rat)

Gene Symbol

NPPA

UniProt

NP_006163.1 (Human)

Additional Atrial Natriuretic Peptide/ANP Products

Product Documents for Atrial Natriuretic Peptide/ANP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Atrial Natriuretic Peptide/ANP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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