Aurora A Antibody (35C1)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1641
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Bovine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 35C1
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant Aurora-A
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Bovine reactivity reported in scientific literature (PMID: 26168150)
Specificity
Recognizes human Aurora-A kinase, a ~46kDa serine/threonine kinase involved in mitotic spindle assembly. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.
Marker
Mitosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aurora A Antibody (35C1)
![Western Blot: Aurora A Antibody (35C1)BSA Free [NB100-1641]](https://resources.bio-techne.com/images/products/Aurora-A-Antibody-35C1-Western-Blot-NB100-1641-img0002.jpg "Western Blot: Aurora A Antibody (35C1)BSA Free [NB100-1641]")
Western Blot: Aurora A Antibody (35C1)BSA Free [NB100-1641]
Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.Applications for Aurora A Antibody (35C1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aurora A
Additional Aurora A Products
Product Documents for Aurora A Antibody (35C1)
Product Specific Notices for Aurora A Antibody (35C1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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