Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Aurora A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790).

Specificity

This is specific for human STK15, Serine/Threonine Kinase 15, Aurora 2, BRAK, ARK1, Aurora-Related Kinase 1

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora A Antibody

Western Blot: Aurora A Antibody [NB100-267]

Western Blot: Aurora A Antibody [NB100-267]

Western Blot: Aurora A Antibody [NB100-267] - Samples: Whole cell lysate (50 ug) from HeLa and HEPG2cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Aurora A Antibody [NB100-267]

Immunoprecipitation: Aurora A Antibody [NB100-267]

Immunoprecipitation: Aurora A Antibody [NB100-267] - Detection of human Aurora A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aurora A antibody NB100-267 used for IP at 3 ug per reaction. Aurora A was also immunoprecipitated by another rabbit anti-Aurora A antibody. For blotting immunoprecipitated Aurora A, NB100-267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Aurora A Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Aurora A

Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Entrez Gene IDs

6790 (Human)

Gene Symbol

AURKA

UniProt

O14965

Additional Aurora A Products

Product Documents for Aurora A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aurora A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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