Aurora A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90103

Novus Biologicals, part of Bio-Techne
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NBP1-90103
NBP1-90103-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aurora A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYL

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aurora A Antibody

Western Blot: Aurora A Antibody [NBP1-90103]

Western Blot: Aurora A Antibody [NBP1-90103]

Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NBP1-90103]

Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NBP1-90103]

Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.
Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103]

Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103]

Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.
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Applications for Aurora A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A

The protein encoded by the Aurora A gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Gene Symbol

AURKA

UniProt

A3KFJ0 (Human)

Additional Aurora A Products

Product Documents for Aurora A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aurora A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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