Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B1

Format

BSA Free

Concentration

1 mg/ml

View all Aurora B Antibodies »

Product Specifications

Immunogen

Recombinant human Aurora B (1-344aa) purified from E. coli

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Aurora B Antibody (2B1) - BSA Free

Western Blot: Aurora B Antibody (2B1)BSA Free [NBP1-74027]

Western Blot: Aurora B Antibody (2B1)BSA Free [NBP1-74027]

Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B
Flow Cytometry: Aurora B Antibody (2B1) - BSA Free [NBP1-74027]

Flow Cytometry: Aurora B Antibody (2B1) - BSA Free [NBP1-74027]

Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for Aurora B Antibody (2B1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora B

The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Gene Symbol

AURKB

UniProt

NP_004208 (Human)

Additional Aurora B Products

Product Documents for Aurora B Antibody (2B1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aurora B Antibody (2B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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