Aurora B Antibody (3F11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50039

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Equine

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Proximity Ligation Assay, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3F11

Concentration

1 mg/ml

View all Aurora B Antibodies »

Product Specifications

Immunogen

Full length recombinant human aurora B protein expressed in and purified from E. coli.

Specificity

This antibody does not cross-react with Aurora A or Aurora C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora B Antibody (3F11)

Western Blot: Aurora B Antibody (3F11) [NBP2-50039]

Western Blot: Aurora B Antibody (3F11) [NBP2-50039]

Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - High magnification confocal immunofluorescence of HeLa cells stained with NBP2-50039, diluted 1:1,000 in green and counterstained with antibody to vimentin at 1:1,000 dilution in red. Blue is the DNA stain DAPI revealing the nuclei. The MCA-3F11 reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - HeLa cell cultures were stained with NBP2-50039 (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.

Applications for Aurora B Antibody (3F11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
Use in PLA reported in scientific literature (PMID:35853811).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora B

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Gene Symbol

AURKB

UniProt

Q96GD4

Additional Aurora B Products

Product Documents for Aurora B Antibody (3F11)

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Product Specific Notices for Aurora B Antibody (3F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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