Aurora B Antibody (4E5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26486
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4E5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Aurora B [UniProt Q96GD4]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aurora B Antibody (4E5)
Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] -
Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] -
Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] -
Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for Aurora B Antibody (4E5)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Aurora B
Alternate Names
Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5
Gene Symbol
AURKB
Additional Aurora B Products
Product Documents for Aurora B Antibody (4E5)
Product Specific Notices for Aurora B Antibody (4E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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