Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Aurora B Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 344) of human Aurora B kinase using the numbering given in Swiss-Prot entry Q96GD4 (GeneID 9212).

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aurora B Antibody

Western Blot: Aurora B Antibody [NB500-185]

Western Blot: Aurora B Antibody [NB500-185]

Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody [NB500-185]

Immunocytochemistry/ Immunofluorescence: Aurora B Antibody [NB500-185]

Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB500-185] - Asynchronous HeLa cells. Antibody used at 0.5 ug/ml in A and at 2 ug/ml in B. Detection : Secondary antibodies were anti-Rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.
Immunoprecipitation: Aurora B Antibody [NB500-185]

Immunoprecipitation: Aurora B Antibody [NB500-185]

Immunoprecipitation: Aurora B Antibody [NB500-185] - Detection of human Aurora B by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 (lot NB500-185-2) used at 0.1 ug/ml. Aurora B was also immunoprecipitated by a previous lot of this antibody (lot NB500-185-1). Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Aurora B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Aurora B

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004).[supplied by OMIM]

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Entrez Gene IDs

9212 (Human)

Gene Symbol

AURKB

UniProt

Q96GD4

Additional Aurora B Products

Product Documents for Aurora B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aurora B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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