Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Aurora C Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human AURKC expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora C Antibody (OTI2B7) - Azide and BSA Free

Western Blot: Aurora C Antibody (OTI2B7)Azide and BSA Free [NBP2-71303]

Western Blot: Aurora C Antibody (OTI2B7)Azide and BSA Free [NBP2-71303]

Western Blot: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aurora C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aurora C.
Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303]

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303]

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded pancreas tissue using anti-Aurora C mouse monoclonal antibody.
Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303]

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303]

Immunohistochemistry: Aurora C Antibody (OTI2B7) - Azide and BSA Free [NBP2-71303] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aurora C mouse monoclonal antibody.
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Applications for Aurora C Antibody (OTI2B7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aurora C

Aurora-C is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Studies show that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger proteins. INCENP binds and activates Aurora-C in vivo and in vitro. Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells (2). Over-expression of an inactive mutant of Aurora C impaired the localization of Aurora C to the spindle midzone resulting in multinucleation which demonstrates that Aurora C is required for cytokinesis (3).

Long Name

Aurora kinase C

Alternate Names

AIE2, AIK3, AIRK3, ARK-3, ARK3, AURKC, Aurora 3, EC 2.7.11.1, STK13

Gene Symbol

AURKC

Additional Aurora C Products

Product Documents for Aurora C Antibody (OTI2B7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aurora C Antibody (OTI2B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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