Autoimmune Regulator/AIRE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1063

Novus Biologicals, part of Bio-Techne
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NB100-1063
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Autoimmune Regulator/AIRE Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QSMARPAAPFPS corresponding to C-Terminus according to NP_000374.1, NP_000649.1.

Localization

Cytoplasmic and Nuclear

Specificity

This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Autoimmune Regulator/AIRE Antibody

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunocytochemistry/ Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Antibody (2 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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Applications for Autoimmune Regulator/AIRE Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 2 ug/mL
Application Notes
ChIP: A customer has reported successful usage (no data). WB: Approx. 60 kDa band observed in human lymph node and human spleen lysates (calculated MW of 57.7 kDa band according to NP_000374.1). An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Autoimmune Regulator/AIRE

The function of the protein is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Entrez Gene IDs

326 (Human)

Gene Symbol

AIRE

UniProt

NP_000374 (Human)

Additional Autoimmune Regulator/AIRE Products

Product Documents for Autoimmune Regulator/AIRE Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Autoimmune Regulator/AIRE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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