Avi Tag Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10546
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Multi-Species
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2623F
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human OX40/TNFRSF4 Avi-tag
Specificity
Detects proteins with Avi Tag in direct ELISAs and Western blots. In Western blots, no cross-reactivity with proteins with His Tag is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Avi Tag Antibody
Detection of Avi Tag by Western Blot.
Western blot shows recombinant human VEGF-165 Avi Tag protein and recombinant human OX40 His Tag protein (negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for recombinant human VEGF-165 Avi Tag protein at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Avi Tag by Simple WesternTM.
Simple Western lane view shows recombinant OX40 Avi Tag protein, loaded at 0.2 mg/mL. A specific band was detected for recombinant OX40 Avi Tag at approximately 74 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Avi Tag Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant human VEGF-165 Avi Tag protein
Sample: Recombinant human VEGF-165 Avi Tag protein
Neutralization
20 µg/mL
Reviewed Applications
Read 1 review rated 5 using MAB10546 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Avi Tag
Alternate Names
Avi Epitope Tag, AviTag
Additional Avi Tag Products
Product Documents for Avi Tag Antibody
Product Specific Notices for Avi Tag Antibody
For research use only
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