Axin-1 Antibody (2B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00008312-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

AXIN1 (NP_003493, 643 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRA

Specificity

AXIN1 - axin 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Axin-1 Antibody (2B11)

Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody (2B11) [H00008312-M01]

Immunocytochemistry/Immunofluorescence: Axin-1 Antibody (2B11) [H00008312-M01] - Analysis of monoclonal antibody to AXIN1 on HeLa cell . Antibody concentration 10 ug/ml.

Proximity Ligation Assay: Axin-1 Antibody (2B11) [H00008312-M01]

Proximity Ligation Assay: Axin-1 Antibody (2B11) [H00008312-M01] - Analysis of protein-protein interactions between GSK3B and AXIN1. HeLa cells were stained with anti-GSK3B rabbit purified polyclonal 1:1200 and anti-AXIN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: Axin-1 Antibody (2B11) [H00008312-M01] - Detection limit for recombinant GST tagged AXIN1 is approximately 0.3ng/ml as a capture antibody.

Applications for Axin-1 Antibody (2B11)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Axin-1

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein), beta 1, 88kDa, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Axis Inhibitor 1

Alternate Names

Axin1, MGC52315

Entrez Gene IDs

8312 (Human)

Gene Symbol

AXIN1

UniProt

NP_003493 (Human)

Additional Axin-1 Products

Product Documents for Axin-1 Antibody (2B11)

Product Datasheets

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COA

SDS

Product Specific Notices for Axin-1 Antibody (2B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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