Axin-1 Antibody (PSH03-00)

Western Blot: Axin-1 Antibody (PSH03-00) [NBP3-32064]

Western Blot: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Western blot analysis of Axin-1 on different lysates with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution.

Lane 1: SW480 cell lysate (10 ug/Lane)
Lane 2: HeLa cell lysate (10 ug/Lane)
Lane 3: 293T cell lysate (10 ug/Lane)
Lane 4: K-562 cell lysate (10 ug/Lane)
Lane 5: Huh7 cell lysate (10 ug/Lane)

Predicted band size: 95.6 kDa
Observed band size: 110 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32064) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Axin-1 Antibody (PSH03-00) [NBP3-32064]

Immunohistochemistry: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32064) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.