Axin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18243
![Western Blot: Axin-1 Antibody [NBP3-18243]](https://resources.bio-techne.com/images/products/Axin-1 Antibody-Western Blot-NBP3-18243-img0003.jpg "Western Blot: Axin-1 Antibody [NBP3-18243]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Summary for Axin-1 Antibody
Immunogen
Synthetic peptide from the mid-protein of Human Axin-1 (aa. 470-570)
Localization
Cytoplasm|Nucleus|Membrane|Cell Membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Axin-1 Antibody
Western Blot: Axin-1 Antibody [NBP3-18243]
Western Blot: Axin-1 Antibody [NBP3-18243] - Western blot analysis of Rat Kidney showing detection of ~95 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18243). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18243) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95 kDa.![Immunohistochemistry: Axin-1 Antibody [NBP3-18243]](https://resources.bio-techne.com/images/products/Axin-1-Antibody-Immunohistochemistry-NBP3-18243-img0002.jpg "Immunohistochemistry: Axin-1 Antibody [NBP3-18243]")
Immunohistochemistry: Axin-1 Antibody [NBP3-18243]
Immunohistochemistry: Axin-1 Antibody [NBP3-18243] - Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.![Immunohistochemistry: Axin-1 Antibody [NBP3-18243]](https://resources.bio-techne.com/images/products/Axin-1-Antibody-Immunohistochemistry-NBP3-18243-img0001.jpg "Immunohistochemistry: Axin-1 Antibody [NBP3-18243]")
Immunohistochemistry: Axin-1 Antibody [NBP3-18243]
Immunohistochemistry: Axin-1 Antibody [NBP3-18243] - Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.Applications for Axin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS pH 7.4, 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Axin-1
Long Name
Axis Inhibitor 1
Alternate Names
Axin1, MGC52315
Gene Symbol
AXIN1
Additional Axin-1 Products
Product Documents for Axin-1 Antibody
Product Specific Notices for Axin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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