Axin-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76526

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Axin-2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. The immunogen is located within amino acids 780 - 830 of AXIN2. Amino Acid Squence: KEQLSKKGNYRYYFKKASDE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken: (85%).

Specificity

Axin-2 antibody is not predicted to cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85, 93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Axin-2 Antibody - BSA Free

Western Blot: Axin-2 AntibodyBSA Free [NBP1-76526]

Western Blot: Axin-2 AntibodyBSA Free [NBP1-76526]

Western Blot: Axin-2 Antibody [NBP1-76526] - Mouse lung lysate at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: Axin-2 Antibody - BSA Free [NBP1-76526]

Immunocytochemistry/ Immunofluorescence: Axin-2 Antibody - BSA Free [NBP1-76526]

Immunocytochemistry/Immunofluorescence: Axin-2 Antibody [NBP1-76526] - HepG2 cells with AXIN2Antibody at 20 ug/ml.
Immunohistochemistry: Axin-2 Antibody - BSA Free [NBP1-76526]

Immunohistochemistry: Axin-2 Antibody - BSA Free [NBP1-76526]

Immunohistochemistry: Axin-2 Antibody [NBP1-76526] - Mouse lung tissue antibody at 5 ug/mL.
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Applications for Axin-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Axin-2

Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Long Name

Axis Inhibitor 2

Alternate Names

AXIL, Axin2, Conductin, MGC10366, MGC126582

Gene Symbol

AXIN2

UniProt

AAF22799 (Human)

Additional Axin-2 Products

Product Documents for Axin-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Axin-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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