Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Axin-2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the mid-protein of Human Axin-2 (aa. 200-300)

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Axin-2 Antibody

Western Blot: Axin-2 Antibody [NBP3-18241]

Western Blot: Axin-2 Antibody [NBP3-18241]

Western Blot: Axin-2 Antibody [NBP3-18241] - Western blot analysis of Rat Lung showing detection of ~93.5 kDa Axin-2 protein using Rabbit Anti-Axin-2 Polyclonal Antibody (NBP3-18241). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Lung. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-2 Polyclonal Antibody (NBP3-18241) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~93.5 kDa.
Immunohistochemistry: Axin-2 Antibody [NBP3-18241]

Immunohistochemistry: Axin-2 Antibody [NBP3-18241]

Immunohistochemistry: Axin-2 Antibody [NBP3-18241] - Immunohistochemistry analysis using Rabbit Anti-Axin-2 Polyclonal Antibody (NBP3-18241). Tissue: Lung Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin-2 Polyclonal Antibody (NBP3-18241) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.

Applications for Axin-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS pH 7.4, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Axin-2

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. (provided by RefSeq)

Long Name

Axis Inhibitor 2

Alternate Names

AXIL, Axin2, Conductin, MGC10366, MGC126582

Gene Symbol

AXIN2

Additional Axin-2 Products

Product Documents for Axin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Axin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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