B220/CD45R Antibody (HIS24)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43408

Novus Biologicals, part of Bio-Techne
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NBP1-43408-0.025mg
NBP1-43408
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # HIS24

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all B220/CD45R Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD45R.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for B220/CD45R Antibody (HIS24)

Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408]

Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408]

Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).
Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408]

Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408]

Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408] - Analysis using the FITC conjugate of NBP1-43408. Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of Anti-Rat CD45R (B220) FITC (filled histogram). Total viable cells were used for analysis.

Applications for B220/CD45R Antibody (HIS24)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B220/CD45R

The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (HIS24)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B220/CD45R Antibody (HIS24)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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