B220/CD45R Antibody (HIS24)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43408
![Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-HIS24-Immunohistochemistry-NBP1-43408-img0004.jpg "Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # HIS24
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen for this antibody was CD45R.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for B220/CD45R Antibody (HIS24)
Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408]
Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).![Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-HIS24-Flow-Cytometry-NBP1-43408-img0003.jpg "Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408]")
Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408]
Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408] - Analysis using the FITC conjugate of NBP1-43408. Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of Anti-Rat CD45R (B220) FITC (filled histogram). Total viable cells were used for analysis.Applications for B220/CD45R Antibody (HIS24)
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Western Blot
1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: B220/CD45R
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (HIS24)
Product Specific Notices for B220/CD45R Antibody (HIS24)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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