B220/CD45R Antibody (IVA103) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-492

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Bovine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # IVA103

Format

BSA Free

Concentration

1 mg/ml

View all B220/CD45R Antibodies »

Product Specifications

Immunogen

Bovine leukocytes.

Specificity

The antibody IVA103 reacts with CD45R antigen (bovine), a single chain type I transmembrane glycoprotein (member of the protein tyrosine phosphatase family), a restricted form of the CD45 family. CD45R is an essential regulator of T and B lymphocytes antigen receptor signaling and it is specifically expressed in hematopoietic cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for B220/CD45R Antibody (IVA103) - BSA Free

Immunocytochemistry: B220/CD45R Antibody (IVA103) - BSA Free [NB500-492]

Immunocytochemistry: B220/CD45R Antibody (IVA103) - BSA Free [NB500-492]

Immunocytochemistry: B220/CD45R Antibody (IVA103) [NB500-492] - Staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD45R (IVA103); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).
B220/CD45R Antibody (IVA103) - BSA Free Flow Cytometry: B220/CD45R Antibody (IVA103) - BSA Free [NB500-492]

Flow Cytometry: B220/CD45R Antibody (IVA103) - BSA Free [NB500-492]

Surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) purified antibody (concentration in sample 0,1 ug/ml) GAM APC.

Applications for B220/CD45R Antibody (IVA103) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunoprecipitation

1:50
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B220/CD45R

CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (IVA103) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B220/CD45R Antibody (IVA103) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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