B220/CD45R Antibody (PTPRC/1461)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53174
![Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1461-Immunocytochemistry-Immunofluorescence-NBP2-53174-img0004.jpg "Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # PTPRC/1461
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human B-lymphocytes (Uniprot: P08575)
Localization
Cell surface and Cytoplasmic
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54464)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for B220/CD45R Antibody (PTPRC/1461)
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]
Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with B220/CD45R Antibody (PTPRC/1461) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1461-Immunohistochemistry-Paraffin-NBP2-53174-img0005.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]")
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Monoclonal Antibody (PTPRC/1461). Image using the Azide and BSA Free format of this antibody.![Flow Cytometry: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]](https://resources.bio-techne.com/images/products/B220-CD45R-Antibody-PTPRC-1461-Flow-Cytometry-NBP2-53174-img0003.jpg "Flow Cytometry: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]")
Flow Cytometry: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174]
Flow Cytometry: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using B220/CD45R Antibody (PTPRC/1461) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).Applications for B220/CD45R Antibody (PTPRC/1461)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: B220/CD45R
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (PTPRC/1461)
Product Specific Notices for B220/CD45R Antibody (PTPRC/1461)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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