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B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12168

Novus Biologicals, part of Bio-Techne
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NBP2-12168
NBP2-12168-0.025mg
Conjugate
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0063-9F14

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Mouse leukemia virus-induced pre-B tumor cells

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free

Immunohistochemistry-Paraffin: B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free [NBP2-12168]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free [NBP2-12168]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (RM0063-9F14) [NBP2-12168] - Staining of paraffin sections of mouse spleen from LPS exposed animals.
Immunohistochemistry: B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free [NBP2-12168]

Immunohistochemistry: B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free [NBP2-12168]

Immunohistochemistry: B220/CD45R Antibody (RM0063-9F14) [NBP2-12168] - Negative control (left) and rat anti mouse CD45R (right).

Applications for B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.4 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B220/CD45R

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B220/CD45R Antibody (RM0063-9F14) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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