B3GNT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99178

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all B3GNT3 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human B3GNT3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for B3GNT3 Antibody

Western Blot: B3GNT3 Antibody [NBP2-99178]

Western Blot: B3GNT3 Antibody [NBP2-99178]

Western Blot: B3GNT3 Antibody [NBP2-99178] - Anti-B3GNT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 60 kDa
Immunoprecipitation: B3GNT3 Antibody [NBP2-99178]

Immunoprecipitation: B3GNT3 Antibody [NBP2-99178]

Immunoprecipitation: B3GNT3 Antibody [NBP2-99178] - B3GNT3 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 ul anti-B3GNT3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-B3GNT3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 64 kDa

Applications for B3GNT3 Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B3GNT3

B3GNT3 encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. [provided by RefSeq]

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 3

Alternate Names

B3GALT8, B3GAL-T8, B3GN-T3, B3GNT-3, Beta-1,3-galactosyltransferase 8, Beta-1,3-GalTase 8, Beta-1,3-Gn-T3, Beta-1,3-N-acetylglucosaminyltransferase 3, beta-1,3-N-acetylglucosaminyltransferase bGnT-3, beta3GalT8, Beta3Gal-T8, Beta3Gn-T3, Beta-3-Gx-T8, BGnT-3, Core 1 extending beta-1,3-N-acetylglucosaminyltransferase, core1-beta3GlcNAcT, EC 2.4.1, EC 2.4.1.-, HP10328, putative type II membrane protein, TMEM3, TMEM3beta3Gal-T8, transmembrane protein 3, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Gene Symbol

B3GNT3

Additional B3GNT3 Products

Product Documents for B3GNT3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B3GNT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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