B4GALT4 Antibody (5E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00008702-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all B4GALT4 Antibodies »

Product Specifications

Immunogen

B4GALT4 (NP_003769, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VGAIQEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPQECKALQRVAILVPHRNRE

Specificity

B4GALT4 - UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for B4GALT4 Antibody (5E2)

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01]

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01]

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 monoclonal antibody (M01), clone 5E2.Lane 1: B4GALT4 transfected lysate(40 KDa).Lane 2: Non-transfected lysate.
Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01]

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01]

Western Blot: B4GALT4 Antibody (5E2) [H00008702-M01] - Analysis of B4GALT4 over-expressed 293 cell line, cotransfected with B4GALT4 Validated Chimera RNAi ( Cat # H00008702-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B4GALT4 monoclonal antibody (M01), clone 5E2 (Cat # H00008702-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: B4GALT4 Antibody (5E2) [H00008702-M01] - Detection limit for recombinant GST tagged B4GALT4 is approximately 0.1ng/ml as a capture antibody.

Applications for B4GALT4 Antibody (5E2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: B4GALT4

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Alternate Names

b4Gal-T4, beta-1,4-galactosyltransferase 4, Beta-1,4-GalTase 4, Beta4Gal-T4, beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4, EC 2.4.1.-, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Entrez Gene IDs

8702 (Human)

Gene Symbol

B4GALT4

OMIM

604015 (Human)

UniProt

NP_003769 (Human)

Additional B4GALT4 Products

Product Documents for B4GALT4 Antibody (5E2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B4GALT4 Antibody (5E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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