Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8B6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all B4GALT4 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 40-322 of human B4GALT4(NP_003769) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B4GALT4 Antibody (OTI8B6) - Azide and BSA Free

Western Blot: B4GALT4 Antibody (OTI8B6)Azide and BSA Free [NBP2-71385]

Western Blot: B4GALT4 Antibody (OTI8B6)Azide and BSA Free [NBP2-71385]

Western Blot: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B4GALT4.
Immunohistochemistry: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385]

Immunohistochemistry: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385]

Immunohistochemistry: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385]

Immunohistochemistry: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385]

Immunohistochemistry: B4GALT4 Antibody (OTI8B6) - Azide and BSA Free [NBP2-71385] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for B4GALT4 Antibody (OTI8B6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B4GALT4

B4GALT4 is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Alternate Names

b4Gal-T4, beta-1,4-galactosyltransferase 4, Beta-1,4-GalTase 4, Beta4Gal-T4, beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4, EC 2.4.1.-, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4

Gene Symbol

B4GALT4

Additional B4GALT4 Products

Product Documents for B4GALT4 Antibody (OTI8B6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for B4GALT4 Antibody (OTI8B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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