B7-H3/CD276 Antibody (PD00-36)

Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138]

Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138]

Flow Cytometry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Flow cytometric analysis of HEK-293 cells labeling B7-H3/CD276.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32138, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).