Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all B7-H3 Antibodies »

Product Summary for B7-H3/CD276 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 484 to 534 of human CD276 Molecule using the numbering given in entry NP_001019907.1 (GeneID 80381).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for B7-H3/CD276 Antibody

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251]

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB
Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251]

Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251]

Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-CD276 antibody NBP2-32251 used for IP at 6 ug per reaction. CD276 was also immunoprecipitated by rabbit anti-CD276 antibody BL16383. For blotting immunoprecipitated CD276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for B7-H3/CD276 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2 - 10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B7-H3

Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Gene Symbol

CD276

UniProt

NP_001019907.1 (Human)

Additional B7-H3 Products

Product Documents for B7-H3/CD276 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for B7-H3/CD276 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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