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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 875023

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Summary for B7-H6 Antibody (875023) - Azide and BSA Free

Immunogen

Partial recombinant human B7-H6 protein (amino acids1-262). [UniProt Q68D85]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for B7-H6 Antibody (875023) - Azide and BSA Free

Flow Cytometry: B7-H6 Antibody (875023) - Azide and BSA Free [MAB71441]

Flow Cytometry: B7-H6 Antibody (875023) - Azide and BSA Free [MAB71441]

Flow Cytometry: B7-H6 Antibody (875023) - Azide Free [MAB71441] - A cell surface stain was performed on Ramos cells with B7-H6 Antibody (875023) MAB71441PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythin.
Flow (Intracellular): B7-H6 Antibody (875023) - Azide and BSA Free [MAB71441]

Flow (Intracellular): B7-H6 Antibody (875023) - Azide and BSA Free [MAB71441]

Flow (Intracellular): B7-H6 Antibody (875023) - Azide Free [MAB71441] - A cell surface stain was performed on U-937 cells with B7-H6 Antibody (875023) MAB71441AF647 (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for B7-H6 Antibody (875023) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B7-H6

B7-H6 is a transmembrane immune regulatory protein that binds to NKp30 expressed on NK cells. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30-dependent cell lysis.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Gene Symbol

NCR3LG1

Additional B7-H6 Products

Product Documents for B7-H6 Antibody (875023) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B7-H6 Antibody (875023) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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