BACE-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77307
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (94%), Guinea Pig (94%), Rat (94%). Backed by our 100% Guarantee.
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE. Amino Acid Squence: CLRQQHDDFADDISLL
Specificity
Human BACE-1 has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD). This antibody detects all human isoforms. Mouse BACE-1 has only 1 isoform (501aa, 55.7kD). Rat BACE-1 has only one isoform identified so far (501aa, 55.8kD).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BACE-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307]
Immunocytochemistry/Immunofluorescence: BACE-1 Antibody [NBP1-77307] - Staining of BACE in 3T3 cells with BACE antibody at 20 ug/ml.Immunohistochemistry: BACE-1 Antibody - BSA Free [NBP1-77307]
Immunohistochemistry: BACE-1 Antibody [NBP1-77307] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.Applications for BACE-1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
10-20 ug/mL
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BACE-1
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Alternate Names
BACE1
Gene Symbol
BACE1
UniProt
Additional BACE-1 Products
Product Documents for BACE-1 Antibody - BSA Free
Product Specific Notices for BACE-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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