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BACE-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77307

Novus Biologicals, part of Bio-Techne
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NBP1-77307
NBP1-77307-0.025mg

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Guinea Pig (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE. Amino Acid Squence: CLRQQHDDFADDISLL

Specificity

Human BACE-1 has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD). This antibody detects all human isoforms. Mouse BACE-1 has only 1 isoform (501aa, 55.7kD). Rat BACE-1 has only one isoform identified so far (501aa, 55.8kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BACE-1 Antibody - BSA Free

Western Blot: BACE-1 AntibodyBSA Free [NBP1-77307]

Western Blot: BACE-1 AntibodyBSA Free [NBP1-77307]

Western Blot: BACE-1 Antibody [NBP1-77307] - IAV via Protein Expression Profile in Cell Lines. Antibodies: BACE, beta-actin, and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.
Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307]

Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307]

Immunocytochemistry/Immunofluorescence: BACE-1 Antibody [NBP1-77307] - Staining of BACE in 3T3 cells with BACE antibody at 20 ug/ml.
Immunohistochemistry: BACE-1 Antibody - BSA Free [NBP1-77307]

Immunohistochemistry: BACE-1 Antibody - BSA Free [NBP1-77307]

Immunohistochemistry: BACE-1 Antibody [NBP1-77307] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.

Applications for BACE-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BACE-1

Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Gene Symbol

BACE1

Additional BACE-1 Products

Product Documents for BACE-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BACE-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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