Bad [p Ser112] Antibody (SR1363)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21589

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21589-100ul
NBP3-21589-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1363

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bad Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-Bad [Ser112] (Uniprot #: Q92934)

Modification

p Ser 112

Specificity

Detects endogenous levels of total phospho-Bad [Ser112]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bad [p Ser112] Antibody (SR1363)

Western Blot: Bad [p Ser 112] Antibody (SR1363) [NBP3-21589] -

Western Blot: Bad [p Ser 112] Antibody (SR1363) [NBP3-21589] - Western blot analysis using NBP3-21589 in (1) HeLa cell lysate; (2) HeLa cell treated with Calcyculin A lysate.

Applications for Bad [p Ser112] Antibody (SR1363)

Application
Recommended Usage

Immunoprecipitation

1:20

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

Additional Bad Products

Product Documents for Bad [p Ser112] Antibody (SR1363)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bad [p Ser112] Antibody (SR1363)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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