Bad Antibody

Western Blot: Bad Antibody [NB100-56080]

Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: Bad Antibody [NB100-56080]

Immunocytochemistry/Immunofluorescence: Bad Antibody [NB100-56080] - Immunofluorescence microscopy of BAD using NB100-56080 at 1:2000. Du145 human prostate carcinoma cells were cultured without (A) or with (B) and (C) 1 uM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr. A and B, staining with BAD antibody, followed by a FITC-conjugated secondary antibody. C, staining with a mitochondrial marker (antibody to mitochondrial Hsp60), followed by a rhodamine-conjugated secondary antibody. THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).

Immunohistochemistry-Paraffin: Bad Antibody [NB100-56080]

Immunohistochemistry-Paraffin: Bad Antibody [NB100-56080] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using 1:2000 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Bad was observed. Staining was performed by Histowiz.