Bad [p Ser112] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23435
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Bad [p Ser112] Antibody
Immunogen
Phospho-Bad (Ser-112) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 112 in mouse Bad. This peptide sequence is highly conserved in human (Ser-75) and rat (Ser-113) Bad. [UniProt# Q61337]
Modification
p Ser112
Specificity
The antibody detects a 23 kDa doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Scientific Data Images for Bad [p Ser112] Antibody
![Bad [p Ser112] Antibody](https://resources.bio-techne.com/images/products/nbp3-23435_rabbit-bad-p-ser112-pab-19920231410131.jpg "Western Blot: Bad [p Ser112] Antibody [NBP3-23435] -")
Western Blot: Bad [p Ser112] Antibody [NBP3-23435] -
Western Blot: Bad [p Ser112] Antibody [NBP3-23435] - Western blot analysis of mouse J774A.1 macrophage stimulated with calyculin A (lanes 1-4) then dephosphorylated with lambda phosphatase (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Bad (lanes 1 & 2), and rabbit polyclonal anti-Bad (Ser-112) (lanes 3 & 4).Applications for Bad [p Ser112] Antibody
Application
Recommended Usage
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Bad
Long Name
Bcl-xL/Bcl-2 Associated Death Promoter
Alternate Names
BBC6, BCL2L8
Gene Symbol
BAD
Additional Bad Products
Product Documents for Bad [p Ser112] Antibody
Product Specific Notices for Bad [p Ser112] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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