Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BAF57 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 360 and the C-terminus (residue 411) of human BAF57/SMARCE1using the numbering given in entry NP_003070.3 (GeneID 6605).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF57 Antibody

Western Blot: BAF57 Antibody [NB100-2591]

Western Blot: BAF57 Antibody [NB100-2591]

Western Blot: BAF57 Antibody [NB100-2591] - Detection of Human and Mouse BAF57/SMARCE1 on HeLa whole cell lysates using NB100-2591.
Immunohistochemistry: BAF57 Antibody [NB100-2591]

Immunohistochemistry: BAF57 Antibody [NB100-2591]

Immunohistochemistry: BAF57 Antibody [NB100-2591] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- BAF57/SMARCE1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for BAF57 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
This antibody is useful for Western Blot and immunoprecipitation. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BAF57

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Alternate Names

BAF57BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, subfamily e, member 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Entrez Gene IDs

6605 (Human); 57376 (Mouse)

Gene Symbol

SMARCE1

UniProt

Q969G3 (Human)

Additional BAF57 Products

Product Documents for BAF57 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BAF57 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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