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BAF57 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31100

Novus Biologicals, part of Bio-Techne
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NBP1-31100

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BAF57. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAF57 Antibody

Western Blot: BAF57 Antibody [NBP1-31100]

Western Blot: BAF57 Antibody [NBP1-31100]

Western Blot: BAF57 Antibody [NBP1-31100] - Raji 7.5% SDS PAGE; antibody diluted at 1:3000
Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-31100]

Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-31100]

Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP1-31100] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAF57 protein stained by BAF57 antibody C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-31100]

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-31100]

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-31100] - Paraffin-embedded lung, using antibody at 1:100 dilution.

Applications for BAF57 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAF57

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Alternate Names

BAF57BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, subfamily e, member 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Gene Symbol

SMARCE1

Additional BAF57 Products

Product Documents for BAF57 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAF57 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.