Bag-1 Antibody (2D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00000573-M02

Novus Biologicals, part of Bio-Techne
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H00000573-M02
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Bag-1 Antibodies »

Product Specifications

Immunogen

BAG1 (NP_004314, 241 a.a. ~ 345 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

BAG1 - BCL2-associated athanogene

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Bag-1 Antibody (2D3)

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02]

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02]

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02]

Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02]

Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on HeLa cell. Antibody concentration 35 ug/ml.
Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02]

Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02]

Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
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Applications for Bag-1 Antibody (2D3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Bag-1

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites.

Long Name

Bcl-2-associated Anthanogene-1

Alternate Names

Bag1

Entrez Gene IDs

573 (Human)

Gene Symbol

BAG1

OMIM

601497 (Human)

UniProt

NP_004314 (Human)

Additional Bag-1 Products

Product Documents for Bag-1 Antibody (2D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bag-1 Antibody (2D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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