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BAIAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-826

Novus Biologicals, part of Bio-Techne
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NB100-826

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for BAIAP2 Antibody

Immunogen

Peptide with sequence C-SMSSADVEVARF corresponding to C-Terminus according to NP_006331.1.

Specificity

This antibody will recognise only one of three reported isoforms (NP_006331.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BAIAP2 Antibody

Western Blot: BAIAP2 Antibody [NB100-826]

Western Blot: BAIAP2 Antibody [NB100-826]

Western Blot: BAIAP2 Antibody [NB100-826] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with EB05175 (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB05175 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: BAIAP2 Antibody [NB100-826]

Immunohistochemistry-Paraffin: BAIAP2 Antibody [NB100-826]

Immunohistochemistry-Paraffin: BAIAP2 Antibody [NB100-826] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for BAIAP2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 2 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human BAIAP2 (myc and DYKDDDDK tagged), a band of approx. 60 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. IHC: Paraffin embedded Human Brain (Cortex).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAIAP2

Brain-specific angiogenesis inhibitor (BAI1)-binding protein may be involved in neuronal growth-cone guidance.

Alternate Names

BAI1-associated protein 2IRS-58, BAI-associated protein 2, BAP2, brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, FLAF3, Insulin receptor substrate p53, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, IRSp53, IRSp53/58, Protein BAP2

Entrez Gene IDs

10458 (Human)

Gene Symbol

BAIAP2

Additional BAIAP2 Products

Product Documents for BAIAP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAIAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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