BAIAP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-826
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for BAIAP2 Antibody
Immunogen
Peptide with sequence C-SMSSADVEVARF corresponding to C-Terminus according to NP_006331.1.
Specificity
This antibody will recognise only one of three reported isoforms (NP_006331.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for BAIAP2 Antibody
Western Blot: BAIAP2 Antibody [NB100-826]
Western Blot: BAIAP2 Antibody [NB100-826] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with EB05175 (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB05175 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: BAIAP2 Antibody [NB100-826]
Immunohistochemistry-Paraffin: BAIAP2 Antibody [NB100-826] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for BAIAP2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
1 - 2 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human BAIAP2 (myc and DYKDDDDK tagged), a band of approx. 60 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. IHC: Paraffin embedded Human Brain (Cortex).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BAIAP2
Alternate Names
BAI1-associated protein 2IRS-58, BAI-associated protein 2, BAP2, brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, FLAF3, Insulin receptor substrate p53, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, IRSp53, IRSp53/58, Protein BAP2
Entrez Gene IDs
10458 (Human)
Gene Symbol
BAIAP2
UniProt
Additional BAIAP2 Products
Product Documents for BAIAP2 Antibody
Product Specific Notices for BAIAP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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