Bax Antibody (6A7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28566

Novus Biologicals, part of Bio-Techne
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NBP1-28566
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Monkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Co-IP, Functional Assay, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A7

Format

Azide and BSA Free

Concentration

0.1 mg/ml

View all Bax Antibodies »

Product Specifications

Immunogen

KLH-conjugated peptide corresponding to amino acids 12-24 located near the N-terminus common to human, mouse and rat Bax.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:34243771) Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Bax Antibody (6A7) - Azide and BSA Free

Western Blot: Bax Antibody (6A7) [NBP1-28566]

Western Blot: Bax Antibody (6A7) [NBP1-28566]

Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY license.
Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566]

Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566]

Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review

Western Blot: Bax Antibody (6A7) [NBP1-28566] -

Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a verified end user.
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Applications for Bax Antibody (6A7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

< = 1 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33143886) Use in functional reported in scientific literature (PMID:31410057). Bax antibody validated for IHC-F, WB from a verified customer reviews.

Reviewed Applications

Read 4 reviews rated 4.8 using NBP1-28566 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Borate buffered saline, pH 8.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Bax

Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (3) Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program. (4-13) The monoclonal antibody 2D2 is specific for human Bax. (1)

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Gene Symbol

BAX

UniProt

Q07812

Additional Bax Products

Product Documents for Bax Antibody (6A7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bax Antibody (6A7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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