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BCAM/CD239 Antibody (BRIC221)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-66523

Clone BRIC221 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-66523
NB100-66523-0.025mg
Conjugate
Catalog #

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # BRIC221

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Human erythrocytes

Reactivity Notes

Predicted cross-reactivities: Porcine

Specificity

NB100-66523 recognizes a non-polymorphic determinant on both the 85 and 78kD Lutheran (Lu) glycoproteins. BRIC 221 specifically recognizes an epitope in the fourth extracellular domain of Lu glycoprotein. Lutheran glycoprotein is a member of the immunoglobulin superfamily and has recently been designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for BCAM/CD239 Antibody (BRIC221)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Non-reducing conditions required in Western Blotting.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCAM

CD239 glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. CD239 is expressed by erythrocytes in the peripheral blood and may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Basal Cell Adhesion Molecule

Alternate Names

CD239

Entrez Gene IDs

4059 (Human)

Gene Symbol

BCAM

UniProt

Additional BCAM Products

Product Documents for BCAM/CD239 Antibody (BRIC221)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BCAM/CD239 Antibody (BRIC221)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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