BCAM/CD239 Antibody (BRIC221) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-66523CL1
Clone BRIC221 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2B Clone # BRIC221
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human erythrocytes
Reactivity Notes
Predicted cross-reactivities: Porcine
Specificity
NB100-66523 recognizes a non-polymorphic determinant on both the 85 and 78kD Lutheran (Lu) glycoproteins. BRIC 221 specifically recognizes an epitope in the fourth extracellular domain of Lu glycoprotein. Lutheran glycoprotein is a member of the immunoglobulin superfamily and has recently been designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for BCAM/CD239 Antibody (BRIC221) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: BCAM
Long Name
Basal Cell Adhesion Molecule
Alternate Names
CD239
Gene Symbol
BCAM
Additional BCAM Products
Product Documents for BCAM/CD239 Antibody (BRIC221) [CoraFluor™ 1]
Product Specific Notices for BCAM/CD239 Antibody (BRIC221) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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