Bcl-2 Antibody (BCL2/6426R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07413
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/6426R) [NBP3-07413]](https://resources.bio-techne.com/images/products/Bcl-2-Antibody-BCL2-6426R-Immunohistochemistry-Paraffin-NBP3-07413-img0001.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/6426R) [NBP3-07413]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BCL2/6426R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein. (Uniprot: P10415 )
Localization
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Specificity
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Marker
Apoptosis& Follicular Lymphoma Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08574)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Bcl-2 Antibody (BCL2/6426R)
Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/6426R) [NBP3-07413]
Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/6426R) [NBP3-07413] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/6426R).Applications for Bcl-2 Antibody (BCL2/6426R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bcl-2
Additional Bcl-2 Products
Product Documents for Bcl-2 Antibody (BCL2/6426R)
Product Specific Notices for Bcl-2 Antibody (BCL2/6426R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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