Bcl-6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31369
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for Bcl-6 Antibody
Immunogen
Bcl6
Reactivity Notes
Chicken (76%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
78.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Bcl-6 Antibody
![Western Blot: Bcl-6 Antibody [NBP2-31369]](https://resources.bio-techne.com/images/products/Bcl6-Antibody-Western-Blot-NBP2-31369-img0003.jpg "Western Blot: Bcl-6 Antibody [NBP2-31369]")
Western Blot: Bcl-6 Antibody [NBP2-31369]
Western Blot: Bcl6 Antibody [NBP2-31369] - WB detection of B-cell lymphoma 6 / Bcl6 protein in (A) lysate of Ramos /human Burkitt's lymphoma cell line and (B) partial recombinant protein using Bcl6 antibody at a concentration of: 2ug/ml for lysate, and 0.5ug/ml for recombinant protein. In Ramos cells, this antibody detected a specific band below ~80 kDa (predicted size 78.9 kDa) and two additional bands ~60kDa/35kDa which are potentially the polyubiquitinization degradation fragments of this protein.![Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-31369]](https://resources.bio-techne.com/images/products/Bcl6-Antibody-Immunohistochemistry-Paraffin-NBP2-31369-img0004.jpg "Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-31369]")
Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-31369]
Immunohistochemistry-Paraffin: Bcl6 Antibody [NBP2-31369] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using Bcl6 antibody at a concentration of 5 ug/ml. Nuclear as well as cytoplasmic immunoreactivity was observed for Bcl6 protein in SCC cells whereas the tumor stroma cells did not develop DAB staining. [40X Magnification]Applications for Bcl-6 Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Bcl-6
Long Name
B Cell Lymphoma/Leukemia 6
Alternate Names
Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51
Gene Symbol
BCL6
UniProt
Additional Bcl-6 Products
Product Documents for Bcl-6 Antibody
Product Specific Notices for Bcl-6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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