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Bcl-6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59786

Novus Biologicals, part of Bio-Techne
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NBP2-59786
NBP2-59786-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acid sequence: NIYSPKETIPEEARSDMHYSVAEGLKPAAPSARNAPYFPCDKASKEEERPSSEDEIALHFEPPNAPLNRKGLVSPQSPQKSDCQPNSPTESCSSKNACILQASGSPPAKSPTDPKACNWKKYKFIVLNS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl-6 Antibody

Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786]

Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786]

Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786] - Staining in human tonsil and pancreas tissues . Corresponding BCL6 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-59786]

Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-59786]

Immunocytochemistry/Immunofluorescence: Bcl-6 Antibody [NBP2-59786] - Human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786]

Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786]

Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786] - Staining of human lymphoma, non-Hodgkin's type, shows strong nuclear positivity in tumor cells.

Applications for Bcl-6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-6

Bcl6 reacts with Bcl6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Long Name

B Cell Lymphoma/Leukemia 6

Alternate Names

Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51

Gene Symbol

BCL6

Additional Bcl-6 Products

Product Documents for Bcl-6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bcl-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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