Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6D1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Bcl G Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 116-216 of human BCL2L14 (NP_620048) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl G Antibody (OTI6D1) - Azide and BSA Free

Western Blot: Bcl G Antibody (OTI6D1)Azide and BSA Free [NBP2-72204]

Western Blot: Bcl G Antibody (OTI6D1)Azide and BSA Free [NBP2-72204]

Western Blot: Bcl G Antibody (OTI6D1) - Azide and BSA Free [NBP2-72204] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2L14 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Immunohistochemistry: Bcl G Antibody (OTI6D1) - Azide and BSA Free [NBP2-72204]

Immunohistochemistry: Bcl G Antibody (OTI6D1) - Azide and BSA Free [NBP2-72204]

Immunohistochemistry: Bcl G Antibody (OTI6D1) - Azide and BSA Free [NBP2-72204] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCL2L14 mouse monoclonal antibody.

Applications for Bcl G Antibody (OTI6D1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl G

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Alternate Names

apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14, BCL2-like 14 (apoptosis facilitator), BCL-G, BCLGbcl2-L-14

Gene Symbol

BCL2L14

Additional Bcl G Products

Product Documents for Bcl G Antibody (OTI6D1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bcl G Antibody (OTI6D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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