Bcl rambo Antibody (Rocky-2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97578

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Rocky-2

Format

BSA Free

Concentration

1.0 mg/ml

View all Bcl rambo Antibodies »

Product Summary for Bcl rambo Antibody (Rocky-2) - BSA Free

Immunogen

Recombinant human Bcl-rambo.

Specificity

Recognizes the C-terminus of human Bcl-rambo (aa 224-459).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Bcl rambo Antibody (Rocky-2) - BSA Free

Immunocytochemistry/ Immunofluorescence: Bcl rambo Antibody (Rocky-2) - BSA Free [NBP1-97578]

Immunocytochemistry/ Immunofluorescence: Bcl rambo Antibody (Rocky-2) - BSA Free [NBP1-97578]

Immunocytochemistry/Immunofluorescence: Bcl rambo Antibody (Rocky-2) [NBP1-97578] - Detection of endogenous Bcl-rambo in HELA cells with the MAb to Bcl-rambo (human) (CT) (Rocky-2). Method: Following fixation with 2% paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection.

Applications for Bcl rambo Antibody (Rocky-2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300

Immunoprecipitation

1:100

Western Blot

1:1000
Application Notes
Detects endogenous protein by Immunocytochemistry, Immunoprecipitation and Western Blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl rambo

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.

Alternate Names

Bcl2-L-13, BCL2-like 13 (apoptosis facilitator), bcl-2-like protein 13, Bcl-rambo, MIL1BCL-RAMBO, Protein Mil1

Gene Symbol

BCL2L13

Additional Bcl rambo Products

Product Documents for Bcl rambo Antibody (Rocky-2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bcl rambo Antibody (Rocky-2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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